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Muhammd Nadeem Hafeez

Researcher at Center for Excellence in Molecular Biology (CEMB), Lahore, Pakistan

Biography

Nadeem Hafeez from Lahore, Pakistan. Currently, I am working as Researcher at Center for Excellence in Molecular Biology (CEMB), Lahore, Pakistan. I have expertise in gene editing, cloning and plant transformation; I have developed a first triple gene genetically engineered cotton variety of Pakistan, which confers significant resistance against biotic and abiotic stresses. I have utilized E.coli and yeast expression models to screen mutated genes before transformation in plants. I am currently doing a research project in abiotic tolerance of cotton to learn more about reverse genetics. In this project we are using CRISPR cas9 genome editing to study genes associated with abiotic stress tolerance.

Abstract

Abstract : Hertrologous expression of uncharacterized wild type and mutated Universal Stress Protein-2 (USP-2) gene from Gossypium arboreum-FDH-171 confers osmotic and salt resistance to Pichia pastoris and Escherichia coli