Applied Microbiology

This study includes hot meal samples from six hotels (10 samples for each). The food samples were identified by planting into Braid Parker Agar including pre-prepared egg-yolk tellurite emulsion. Isolated S. aureus bacteria were analyzed for enterotoxin via the commercial kit of Reversed Passive Latex Agglutination. 21 out of 60 meal samples were found S. aureus contaminated in 1.0×103- 4.7×104 kob/g. Enterotoxin was found positive in 6 samples out of 12 in which the S. aureus number was detected more than 1.0×103.In the samples of the first hotel, one SEB was found positive. In the samples of the second hotel, one SEC was found positive. In the samples of the third hotel, one SEC was found positive. In the samples of the forth hotel, no enterotoxin was found positive. In the samples of the sixth hotel, one SEA and one SED were found positive. In inspected 21 S. aureus bacteria, enterotoxin A (SEA) was found positive in one of the samples (10%), enterotoxin B (SEB) was found positive in two samples (5%), enterotoxin C (SEC) was found positive in two food samples (5%), enterotoxin D (SED) was also found positive in one food samples (6.7%). In hotels having all inclusive system, hot meals require extra attention for food safety. Heat-processing is not effective in terms of food intoxication.To protect consumer health in a better way, it is vital to pay extra attention to general sanitation rules and to handling food safety like

HACCP and to staff hygiene.