Applied Microbiology

Pseudomonas aeruginosa is an important opportunistic pathogen that plays an important role in hospital and causes a wide spectrum of nosocomial infections that can lead to sepsis, pneumonia, endocarditis and urinary tract infections. The development of resistance of P. aeruginosa to antibiotics is increasing globally due to the overuse of antibiotics. This study examines the antibiotic

resistance in clinical isolates of P. aeruginosa that was carried out in the Central Bacteriology Laboratory, University Hospital Center in Batna from January 2015 to December 2015. All samples received in the laboratory were processed according to Clinical and Laboratory Standards Institute (CLSI, 2014) guidelines. Identification of P. aeruginosa was done by conventional (API 20 NE)techniques and antimicrobial susceptibility pattern to 14 antimicrobial agents was determined by disc diffusion method. A total of 199 patients with mean age 245±22.15 (50.25% males and 49.75% females) were included. The resistance was seen with, levofloxacin(21.6%), ceftazidime (9.55%), piperacillin (38.68%), colistin (6.03%), gentamicin (30.15%), ciprofloxacin (9.04%) aztreonam (8.54%) and imipenem (14.07%). The most frequent mechanisms of resistance were OprD2 (2.01%) and metallo-b-lactamase MβL (1%). The most affected departments were: Burned (29.14%), neurosurgery (17.58%) and the medical-ICU (11.55%). Specimen’s frequency according to the isolation rate was: Pus (53.77%) and cerebrospinal fluid (12.56%). In the present study aztreonam, ceftazidime,ciprofloxacin and colistin were found to be the most effective drugs against P. aeruginosa.