Tatyana Tracevska
University of Latvia, Latvia
Title: Interdisciplinary studies on Ayurvedic herbal formulation showing antibacterial effect on methicillin resistant Staphylococcus aureus
Biography
Biography: Tatyana Tracevska
Abstract
Statement of the Problem: Emerging bacterial resistance in response to antimicrobial therapy is a world-wide problem nowadays, stimulating the search for new antimicrobial compounds. Combinatory therapy including antibacterial agents and herbal extracts, is able to combat bacterial resistance, is the promising perspective. The object of present study is a polyherbal formulation, Jathyadi Thailam, based on 13 herb infusion in coconut oil, used in Ayurvedic medicine for chronic wound (e.g. Diabetic foot ulcers) and burns healing.
Methodology & Theoretical Orientation: The antimicrobial activity of this formulation for biofilm forming and drug resistant bacteria such as methicillin resistant staphylococci, or extended spectrum beta-lactamase producing bacilli has been studied. The antibacterial efficacy of the herbal oil and its polar and nonpolar crude extracts was tested by agar dilution method and by broth microdilution method for mostly common isolates from diabetic wounds. Reference strains and clinical isolates from diabetic foot ulcers isolated in Latvian hospital were analyzed.
Findings: Interestingly, the bacteriostatic effect was shown by Jathyadi Thailam by the agar dilution method for methicillin resistant Staphylococcus aureus ATCC 38592, drug susceptible S.aureus ATCC 2848 and clinical S.aureus strain, Pseudomonas aeruginosa ATCC 2843, Enterococcus faecalis ATCC 29212 and biofilm producing S.epidermidis reference strains. No inhibition was observed for Klebsiella pnemoniae ATCC 2558, Proteus mirabilis ATCC 432351, Echerichia coli ATCC 25922 and for clinical Prowidencia spp. and ESBL producing K.pneumoniae strain. Minimal bactericidal concentration (MBC) was determined for the crude herbal polar and nonpolar extracts by the broth microdilution method. The MBC was determined as 15.6 mg/ml and 31.2 mg/ml by polar and nonpolar extracts, respectively, for MRSA ATCC 38592, 7.8 mg/ml by both extracts for S.aureus ATCC 2848, 1.95 mg/ml by both extracts for biofilm producing S.epidermidis and 62.5mg/ml by nonpolar extract for P. aeruginosa.
Conclusion & Significance: To conclude, both herbal crude extracts and the final herbal oil were found to be more effective for Gram positive bacteria, when for Gram negative. The formulation was effective against Staphyloccus aureus, the bacteria predominant in chronic diabetic wounds. Further studies will be focused on development of herbal suspension with better skin penetrating properties and aimed to inhibit the growth of Gram negative bacteria.
Recent Publications
- Dowd SE, Sun Y, Secor PR, Rhoads DD, Wolcott BM et al. (2008) Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing. BMC Microbiol 8: 43.
- Dhande Priti P, Raj S, Kureshee N et al. (2012) Burn wound healing potential of Jatyadi formulations in rats. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 3(4): 747-754.
- Boutoille D, Fe Ìraille A, Maulaz D et al (2008) Quality of life with diabetes-associated foot complications: comparison between lower-limb amputation and chronic foot ulceration. Foot and Ankle International, 29(11):1074–1078.
- Payne D, Gwynn M, Holmes DJ et al (2007) Drugs for bad bugs: confronting the challenges of antibacterial discovery. Nature Reviews Drug Discovery 6(1):29– 40.
- Menon SS, Pednekar S and Singh A (2011) Wound healing efficacy of Jatyadi Taila: in vivo evaluation in rat using excision wound model, J. Ethnopharmacol. 138: 99-104.