Neslihan Dikbas
Ataturk University, Turkey
Title: Purification and characterization of a beta-mannanase from Lactobacillus plantarum
Biography
Biography: Neslihan Dikbas
Abstract
Thermoalkaline mannanase enzyme from Lactobacillus plantarum (X) bacterium was purified for 111 folds and with 36% yield by utilizing ammonium sulfate sedimentation technique along with DEAE-Sephadex ion exchange chromatography and Sephacryl S200 Gel filtration chromatography. It had been observed that the enzyme was formed of two subunits; 35 kDa and 55 kDa when purified enzyme was carried out in gel filtration chromatography and SDS PAGE electrophoresis systems. Optimum temperature was set out as 40 oC whereas optimum pH was determined to be 10. It has been observed that the activity of the enzyme was stable between the ranges of pH levels of 3-11 and at the temperature of 90 oC and did not lose its activity. Additionally, the effect of the several metal ions such as Ca2+, Mn2+, Co2+, Zn2+, Cu2+, Fe2+ and Ni2+ on the enzyme activity was tried out and it was observed that these mentioned metal ions increased the activity of the enzyme by 100-344%. Furthermore, the purified enzyme was tested in order to investigate the activity of this enzyme on the clarification of some fruit juice such as orange, apricot, grape and apple juice. During the process of comparison with crude extract, the highest amount of purified enzyme was detected in apple juice with the percentage of 154%.