Zehranur Yuksekdag has completed her PhD from Gazi University, Turkey and currently working as a Professor Doctor in the same university. Her areas of expertise are probiotics, microbial biotechnology and food microbiology. She has published more than 27 papers in reputed journals and serving as an Editorial Board Member, Referees in different reputed journals and has worked in 22 research projects.
β-glucosidase, responsible for hydrolysis of cellulose is an enzyme that are produced by microorganisms and changes isoflavone glucosidases into the form of bioactive isoflavone aglycones, which are beneficial in terms of health. β-glucosidase has a commercially important role with its usage in various fields such as; biotechnology, food industry and pharmacology. It is quite efficient in prevention of various chronic diseases such as cancer, because it blocks the enzymes that support tumor growth. In this research, 3 strains (originated from human, food and animal) that belong to Lactobacillus species were used. In vitro conditions for simulating gastric and intestinal digestion were designed. β-glucosidase enzyme activities of 3 strains were evaluated by using p-nitrophenyl-β-D glikopiranozit (p-NPG) as a substrate. We observed that in the simulated gastric phase the highest specific enzyme activity occurred at pH level of 4.0 (2.200 U/mg) at Lactobacillus casei SC1 while the lowest specific enzyme activity at L. rhamnosus MBA9 occurred at pH level of 2.0 (0.630 U/mg). On the other hand, in the simulated intestinal phase, the highest specific enzyme activity was found to take place at pH 8.0 (1.170 U/mg) at L. casei SC1. In this study, it has been determined that, the bacteria show high β-glucosidase enzyme activity by protecting their aliveness in gastro-intestinal environment. An alternative perspective has been gained for their probiotic characteristics in terms of tolerating the gastric acid environment and the gall in the intestine.
Raniyah Ahmad Maki Shoudri has completed her MSc from King Abdulaziz University in Applied Medical Technology, Clinical Microbiology and Immunology. She is a Laboratory Specialist in Reference and Diagnostic Laboratory, Jeddah, the Ministry of Health.
The first civilization dealt with disorders and provided detailed information about wound management was ancient Egyptians. Wound infections evidently appeared in times of World War I that accounted a significant mortality and morbidity rate among injured soldiers. Currently, around 11 million people worldwide require medical treatment for wound infections and 300,000 people die every year. Scientists highlighted the concept of introducing micro-organisms into wounds resulting in an infection. The infected wound was successfully treated with wide-spectrum antibiotics that can eradicate the pathogenic micro-organisms. Due to extensive use of antimicrobial drugs such as β-lactam, methicillin, vancomycin, etc., new strains with high resistance have been emerged. During the past few decades, nanotechnology has arisen with new promising technology for synthesis of nanobiomaterials. Researchers focused on using nanobiotechnology to employee several nanoparticles as antimicrobial agent. Metallic nanoparticles such as zinc, copper, titanium and silver have demonstrated antibacterial activity. Metallic nanoparticles will be considered as a new alternative treatment, which replaces the existing antibiotics. Antibacterial activity of these nanoparticles, escpicially TiO2 will enhance the future of the therapeutic strategies against the resistant pathogenic strains that cause wound infections.